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LC-MS analysis The samples (SK-N-SH MYCN/GFP) were solubilized using a Bead Beater, then reduced with 10 mM DTT in 0.1 M ammonium bicarbonate and then alkylated with 50 mM iodoacetamide in 0.1 M ammonium bicarbonate (both room temperature for 0.5 h)

LC/MS analysis of HLA class II eluted peptides The samples were analyzed with a Thermo LUMOS mass spectrometer using a Thermo easy 1200 nLC-HPLC system