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Step 04 Lyophilization Partially stoppered vials transfer directly into trays for lyophilization, a controlled freeze-drying cycle that sublimates water under vacuum to preserve the compound and keep it stable at room temperature

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Gut barrier disruption patterns EHS-induced gut barrier dysfunction is primarily driven by splanchnic hypoperfusion (from increased skeletal muscle blood flow during exertion) and mechanical stress (muscle contraction), leading to rapid translocation of LPS and bacteria within 24 hours [61]