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HFD mice were randomly divided into four groups according to the case number and treated with recombinant sFNDC5 (r-sFNDC5) (0.5 g/g), recombinant irisin (r-irisin) (0.5 g/g), liraglutide (1 g/g), or saline by intraperitoneal (i.p.) injection once daily from 9 to 10 a.m