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Consider a 45-degree angle

Indexes were added in a subsequent PCR (98 C for 30 sec, 8 (98 C for 10 s, 55 C for 20 s, 72 C for 20 s), 72 C for 5 min) using the Illumina Nextera Index Kit V2 and the attachments were verified by running the products on an agarose gel